6V7N
Crystal Structure of a human Lysosome Resident Glycoprotein, Lysosomal Acid Lipase, and its Implications in Cholesteryl Ester Storage Disease (CESD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-10 |
Detector | DECTRIS EIGER2 X 500K |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.145, 165.486, 60.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.780 - 2.620 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hlg |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-3000 |
Data scaling software | SCALA |
Phasing software | BUSTER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 97.100 | 2.630 |
High resolution limit [Å] | 2.620 | 2.622 |
Rmerge | 0.135 | 1.361 |
Number of reflections | 30000 | 1991 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 100.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.996 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1.8 M Ammonium sulfate, 0.1 M CH3COONa, pH 5.5 |