6V7C
Human Arginase1 Complexed with Bicyclic Inhibitor Compound 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.980, 281.380, 67.260 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 35.840 - 1.800 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gmz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.420 | 1.811 |
| High resolution limit [Å] | 1.800 | 1.805 |
| Rmerge | 0.110 | 0.510 |
| Number of reflections | 169488 | 1767 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 10% MMT (pH 7.0) 0.1 M ammonium formate, 16-22% PEG 8000 |
