6V6V
Crystal structure of oncogenic RhoA mutant G14V complexed with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.225, 86.924, 34.078 |
| Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
| Resolution | 24.450 - 1.400 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1FTN |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.817 | 25.817 | 1.480 |
| High resolution limit [Å] | 1.400 | 4.430 | 1.400 |
| Rmerge | 0.195 | 0.828 | |
| Rmeas | 0.326 | 0.272 | 1.092 |
| Rpim | 0.168 | 0.142 | 0.654 |
| Total number of observations | 131266 | 4131 | 13015 |
| Number of reflections | 36248 | 1143 | 4904 |
| <I/σ(I)> | 2.8 | 4.4 | 0.9 |
| Completeness [%] | 98.8 | 95.5 | 92.7 |
| Redundancy | 3.6 | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | 20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0 |
