6V6U
Crystal structure of RhoA-GDP with novel Switch I conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.216, 87.192, 34.271 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 34.140 - 1.160 |
| R-factor | 0.1705 |
| Rwork | 0.170 |
| R-free | 0.18460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1FTN |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.190 | 1.220 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.065 | 0.446 |
| Rmeas | 0.091 | 0.624 |
| Rpim | 0.048 | 0.323 |
| Number of reflections | 64753 | 9359 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 277 | 20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0 |
