6V63
SETD3 WT in Complex with an Actin Peptide with His73 Replaced with Glutamine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.344, 175.795, 66.807 |
Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
Resolution | 36.710 - 2.020 |
R-factor | 0.1669 |
Rwork | 0.166 |
R-free | 0.19880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mbj |
RMSD bond length | 0.004 |
RMSD bond angle | 0.638 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.710 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 84534 | 7028 |
<I/σ(I)> | 12.4 | |
Completeness [%] | 92.9 | |
Redundancy | 5.7 | |
CC(1/2) | 0.990 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |