6V5T
Crystal structure of human prethrombin-2 with tryptophans replaced by 5-F-tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-12-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.545, 58.887, 52.455 |
| Unit cell angles | 90.00, 98.42, 90.00 |
Refinement procedure
| Resolution | 38.960 - 2.100 |
| R-factor | 0.1799 |
| Rwork | 0.177 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sqh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.534 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.559 | |
| Number of reflections | 15696 | 1382 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.3 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Hepes pH 7.5, 25% PEG 8000 |
