6V5A

Crystal structure of the human BK channel gating ring L390P mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	145.518, 145.518, 242.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.203
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mt5
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.055	0.734
Number of reflections	67264	6646
<I/σ(I)>	30.6
Completeness [%]	100.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	295	50 mM sodium acetate, 4% (w/v) PEG 4000, 100 mM sodium sulfate, and 100 mM lithium sulfate (pH 4.9)