6V4L
Structure of TrkH-TrkA in complex with ATPgammaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-10-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 |
| Unit cell lengths | 164.271, 164.271, 123.388 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.329 - 3.800 |
| R-factor | 0.256616776797 |
| Rwork | 0.254 |
| R-free | 0.29821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j9u |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 3.870 |
| High resolution limit [Å] | 3.780 | 3.800 |
| Rmerge | 0.073 | 1.560 |
| Number of reflections | 32522 | 1609 |
| <I/σ(I)> | 38.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 293 | 50 mM magnesium chloride, 50 mM ammonium phosphate monobasic, 100 mM HEPES, pH 7.3, 20% PEG400 |
