6V4L

Structure of TrkH-TrkA in complex with ATPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 42
Unit cell lengths	164.271, 164.271, 123.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.329 - 3.800
R-factor	0.256616776797
Rwork	0.254
R-free	0.29821
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j9u
RMSD bond length	0.005
RMSD bond angle	0.938
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	3.870
High resolution limit [Å]	3.780	3.800
Rmerge	0.073	1.560
Number of reflections	32522	1609
<I/σ(I)>	38.9
Completeness [%]	99.8
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	50 mM magnesium chloride, 50 mM ammonium phosphate monobasic, 100 mM HEPES, pH 7.3, 20% PEG400