6V4G
Crystal Structure Analysis of Zebra fish MDMX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.290, 108.930, 30.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.465 - 1.250 |
R-factor | 0.1707 |
Rwork | 0.169 |
R-free | 0.19620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n5t |
RMSD bond length | 0.005 |
RMSD bond angle | 0.826 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.470 | 1.270 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmeas | 0.073 | 1.783 |
Rpim | 0.028 | 0.700 |
Total number of observations | 248825 | 12096 |
Number of reflections | 38363 | 1894 |
<I/σ(I)> | 11.3 | 1.1 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100mM Tris-8.0, 2.4M ammonium sulfate |