6V4E
Crystal Structure Analysis of Zebra Fish MDM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.950, 30.720, 69.260 |
| Unit cell angles | 90.00, 106.89, 90.00 |
Refinement procedure
| Resolution | 54.678 - 1.620 |
| R-factor | 0.1616 |
| Rwork | 0.160 |
| R-free | 0.19850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n5t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.680 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmeas | 0.057 | 1.502 |
| Rpim | 0.031 | 0.825 |
| Total number of observations | 114469 | 4976 |
| Number of reflections | 34317 | 1599 |
| <I/σ(I)> | 12.5 | 1 |
| Completeness [%] | 98.4 | 93.4 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM sodium citrate (pH 5.0), 1.8M sodium malonate |
