6V3Y

Crystal structure of EED in complex with PALI1-K1219me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	57.775, 85.296, 91.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.790 - 1.630
R-factor	0.1665
Rwork	0.165
R-free	0.19250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jzn
RMSD bond length	0.006
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	1.710
High resolution limit [Å]	1.630	1.630
Rmerge	0.074	0.643
Number of reflections	57098	8025
<I/σ(I)>	16.2
Completeness [%]	99.7	97.9
Redundancy	8.1	8.1
CC(1/2)		0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.6 M sodium formate, 10 mM TCEP, 5% glycerol