6V3X

Crystal structure of EED in complex with PALI1-K1241me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	56.243, 84.721, 90.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.240 - 1.700
R-factor	0.175
Rwork	0.174
R-free	0.19990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iiw
RMSD bond length	0.006
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.760	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.066	0.631
Rmeas	0.071	0.674
Rpim	0.025	0.234
Number of reflections	47118	6664
<I/σ(I)>	16.5
Completeness [%]	97.8	96.5
Redundancy	7.6	8
CC(1/2)	0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.6 M sodium formate, 10 mM TCEP, 5% glycerol