6V37
K2P2.1(TREK-1)I110D:RuR:ML335 bound channel structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.018, 118.735, 129.036 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.990 - 2.800 |
R-factor | 0.2735 |
Rwork | 0.272 |
R-free | 0.31650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rue |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 3.180 |
High resolution limit [Å] | 2.800 | 3.000 |
Number of reflections | 25993 | 2070 |
<I/σ(I)> | 13.6 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 | |
CC(1/2) | 0.998 | 0.553 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M KCl, 1-3 mM CdCl2, 0.1 M HEPES pH 7-8, 20-26% PEG400 |