6V2K
The nucleosome structure after H2A-H2B exchange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-24 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.561, 107.711, 168.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.723 - 2.600 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y0c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.095 | 0.063 | 0.501 |
| Rmeas | 0.105 | 0.069 | 0.563 |
| Rpim | 0.043 | 0.029 | 0.252 |
| Total number of observations | 291041 | ||
| Number of reflections | 55188 | 5842 | 5309 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 98.5 | 98.7 | 96.5 |
| Redundancy | 5.3 | 5.7 | 4.4 |
| CC(1/2) | 0.998 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
