6V2K

The nucleosome structure after H2A-H2B exchange

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	98.561, 107.711, 168.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.723 - 2.600
R-factor	0.1987
Rwork	0.197
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y0c
RMSD bond length	0.010
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.095	0.063	0.501
Rmeas	0.105	0.069	0.563
Rpim	0.043	0.029	0.252
Total number of observations	291041
Number of reflections	55188	5842	5309
<I/σ(I)>	6.3
Completeness [%]	98.5	98.7	96.5
Redundancy	5.3	5.7	4.4
CC(1/2)		0.998	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	potassium cacodylate, potassium chloride, manganese chloride