6V08
Crystal structure of human recombinant Beta-2 glycoprotein I (hrB2GPI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 159.327, 173.248, 115.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.580 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c1z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.041 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 2.640 |
| High resolution limit [Å] | 2.580 | 2.600 |
| Rmerge | 0.143 | 0.647 |
| Number of reflections | 48503 | 1919 |
| <I/σ(I)> | 10 | 1.5 |
| Completeness [%] | 97.1 | 77.1 |
| Redundancy | 7.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100 mM MES and 1.6 M AMSO4 |
