6UZT

Crystal Structure of RPTP alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	P 2 21 21
Unit cell lengths	104.720, 112.110, 136.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.1568
Rwork	0.155
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfo
RMSD bond length	0.006
RMSD bond angle	1.476
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	29.940	1.830
High resolution limit [Å]	1.800	9.860	1.800
Rmerge	0.086	0.032	0.877
Rmeas	0.094	0.035	0.966
Rpim	0.037	0.014	0.401
Total number of observations		5926	41573
Number of reflections	148737	1002	7290
<I/σ(I)>	10.6	25.3	1.9
Completeness [%]	100.0	96.7	100
Redundancy	6.2	5.9	5.7
CC(1/2)	0.998	0.999	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	MES, PEG 20000, DTT