6UZT
Crystal Structure of RPTP alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 104.720, 112.110, 136.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.1568 |
Rwork | 0.155 |
R-free | 0.19100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yfo |
RMSD bond length | 0.006 |
RMSD bond angle | 1.476 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 29.940 | 1.830 |
High resolution limit [Å] | 1.800 | 9.860 | 1.800 |
Rmerge | 0.086 | 0.032 | 0.877 |
Rmeas | 0.094 | 0.035 | 0.966 |
Rpim | 0.037 | 0.014 | 0.401 |
Total number of observations | 5926 | 41573 | |
Number of reflections | 148737 | 1002 | 7290 |
<I/σ(I)> | 10.6 | 25.3 | 1.9 |
Completeness [%] | 100.0 | 96.7 | 100 |
Redundancy | 6.2 | 5.9 | 5.7 |
CC(1/2) | 0.998 | 0.999 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MES, PEG 20000, DTT |