6UZR

Crystal structure of GLUN1/GLUN2A ligand-binding domain in complex with glycine and homoquinolinic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92013
Spacegroup name	P 21 21 21
Unit cell lengths	54.367, 88.717, 125.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.360 - 1.870
R-factor	0.1746
Rwork	0.173
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf8
RMSD bond length	0.007
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	1.910
High resolution limit [Å]	1.870	1.870
Rmerge	0.116	0.944
Number of reflections	50584	4950
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM HEPES, pH 7.0, 75 mM sodium chloride, 18% PEG2000 MME