6UYG

Structure of Hepatitis C Virus Envelope Glycoprotein E2c3 core from genotype 6a bound to broadly neutralizing antibody AR3A and non neutralizing antibody E1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 63 2 2
Unit cell lengths	209.824, 209.824, 139.942
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.600 - 3.375
R-factor	0.2564
Rwork	0.254
R-free	0.30550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bkb
RMSD bond length	0.002
RMSD bond angle	0.548
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.460
High resolution limit [Å]	3.375	3.400
Number of reflections	25916	1258
<I/σ(I)>	13.5
Completeness [%]	100.0
Redundancy	17.2
CC(1/2)	0.920	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M magnesium chloride, 10% (w/v) PEG 3000, 15% ethylene glycol, 0.1M Na-cacodylate, pH=6.5