6UXL
Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Forrest complexed with PLP-Glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-03-31 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.727, 127.445, 127.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.412 - 2.350 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uxh |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.412 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.151 | 2.231 |
Rmeas | 0.163 | 2.421 |
Rpim | 0.061 | 0.933 |
Total number of observations | 25177 | |
Number of reflections | 38807 | 3772 |
<I/σ(I)> | 11.2 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.7 |
CC(1/2) | 0.998 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.18 M trimethylamine N-oxide, 0.1 M Tris pH 8.5, 22.5% PEG MME 2000 |