6UXI
Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP-Glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 56.567, 124.547, 129.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.839 - 2.100 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uxh |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.840 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.144 | 1.828 |
Rmeas | 0.157 | 1.986 |
Rpim | 0.061 | 0.767 |
Total number of observations | 353156 | 28628 |
Number of reflections | 54101 | 4371 |
<I/σ(I)> | 10.5 | 1.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.5 |
CC(1/2) | 0.997 | 0.311 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.19 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21% (w/v) PEG MME 2000, 20 mM glycine |