6UX2
Crystal structure of ZIKV RdRp in complex with STAT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2018-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.113, 124.700, 84.783 |
| Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
| Resolution | 49.240 - 3.010 |
| R-factor | 0.2432 |
| Rwork | 0.242 |
| R-free | 0.26350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YVL 5TMH |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.656 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.240 | 3.115 |
| High resolution limit [Å] | 3.008 | 3.008 |
| Number of reflections | 32545 | 2737 |
| <I/σ(I)> | 9.52 | |
| Completeness [%] | 97.9 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.996 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298.15 | 0.2 M ammonium sulfate, 11% PEG 8000 and 0.1 M Tris-HCl, pH 8.5 |
