6UUH

Crystal structure of broad and potent HIV-1 neutralizing antibody 438-B11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	71.157, 72.751, 108.716
Unit cell angles	90.00, 101.17, 90.00

Refinement procedure

Resolution	48.220 - 2.700
R-factor	0.229
Rwork	0.227
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tfs
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rpim	0.050	0.530
Number of reflections	29107	1479
<I/σ(I)>	12.9
Completeness [%]	96.6
Redundancy	3
CC(1/2)	0.890	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.085M sodium acetate (pH=4.0), 0.17M ammonium acetate, 5% %(v/v) glycerol, 27.882 %(w/v) PEG4000, 15% glycerol