6UUB
E. coli sigma-S transcription initiation complex with a mismatching UTP ("Fresh" crystal soaked with UTP for 2 hours)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 132.942, 154.992, 233.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 3.955 |
| Rwork | 0.303 |
| R-free | 0.37460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipl |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.181 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.900 | 4.190 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Number of reflections | 42501 | 6537 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.1 | 95.9 |
| Redundancy | 5 | 4.5 |
| CC(1/2) | 0.999 | 0.146 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG3350, sodium chloride, HEPES |
