6UTA
Crystal structure of Z004 iGL Fab in complex with ZIKV EDIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 85.907, 85.907, 327.458 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.420 - 3.100 |
R-factor | 0.2751 |
Rwork | 0.274 |
R-free | 0.29120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vic |
RMSD bond length | 0.013 |
RMSD bond angle | 1.571 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.420 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rpim | 0.088 | 0.491 |
Number of reflections | 21992 | 1620 |
<I/σ(I)> | 9.1 | 1.7 |
Completeness [%] | 93.6 | 71.4 |
Redundancy | 12.4 | |
CC(1/2) | 0.990 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | % w/v tryptone, 0.001 M sodium azide, 0.05 M HEPES sodium pH 7.0, 20% w/v polyethylene glycol 3,350 |