6UPA

Crystal Structure of GTPase Domain of Human Septin 2/Septin 6 Heterocomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96861
Spacegroup name	P 41 21 2
Unit cell lengths	82.730, 82.730, 170.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.209 - 2.510
R-factor	0.226
Rwork	0.224
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qnr 4kva
Data reduction software	xia2
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.210	37.210	2.580
High resolution limit [Å]	2.510	11.230	2.510
Rmerge	0.152	0.044	2.235
Rmeas	0.159	0.046	2.324
Rpim	0.044	0.014	0.635
Total number of observations	269502	2903	20143
Number of reflections	21021	299	1516
<I/σ(I)>	11.6	37.6	1.2
Completeness [%]	99.9	97.8	99.9
Redundancy	12.8	9.7	13.3
CC(1/2)	0.999	0.999	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	100mM bicine/Trizma base pH 8.5, 12.5% PEG 1000, 12.5% PEG 3350, 12.5% MPD and 20mM of each d-glucose, d-mannose,d-galactose, l-fucose, d-xylose, N-acetyld-glucosamine