6UP3

Crystal structure of the murine DHX36 helicase in complex with ANP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-01-08
Detector	PSI PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	66.446, 116.147, 132.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.847 - 2.691
R-factor	0.2025
Rwork	0.200
R-free	0.25380
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3374)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.847	2.790
High resolution limit [Å]	2.690	2.690
Rmeas	0.100	0.961
Number of reflections	28736	2720
<I/σ(I)>	17.1	2.2
Completeness [%]	99.1
Redundancy	5.3
CC(1/2)	1.000	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	8% PEG8000, 100 mM ammonium acetate, 20 mM magnesium chloride, 50 mM HEPES, pH 7.0, 15% glycerol