6UOT
Cryo-EM structure of the PrgHK periplasmic ring from the Salmonella SPI-1 type III secretion needle complex solved at 3.3 angstrom resolution
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.3 |
Software | RELION,CTFFIND,PHENIX |
Symmetry | C24 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |