6UMI

Crystal structure of erenumab Fab-b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	72.329, 72.329, 163.762
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.1854
Rwork	0.184
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.105	0.061	0.589
Rmeas	0.110	0.064	0.617
Rpim	0.033	0.020	0.183
Total number of observations	215604
Number of reflections	19967	2149	1935
<I/σ(I)>	10.4		5.36
Completeness [%]	99.7	99.2	99.5
Redundancy	10.8	10.3	11.1
CC(1/2)		0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	277	0.1 M sodium acetate, pH 4.0, 22% PEG6000, 10% isopropanol