6UL0
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.382, 61.473, 36.336 |
| Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
| Resolution | 47.610 - 1.760 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4KSY |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data scaling software | STARANISO (1.10.9beta) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.610 | 1.891 |
| High resolution limit [Å] | 1.760 | 1.762 |
| Rmerge | 0.055 | 0.743 |
| Rmeas | 0.060 | 0.496 |
| Rpim | 0.032 | 0.262 |
| Total number of observations | 6920 | |
| Number of reflections | 16734 | 1995 |
| <I/σ(I)> | 12.2 | 2.8 |
| Completeness [%] | 80.5 | 21.3 |
| Redundancy | 3.3 | 2.1 |
| CC(1/2) | 0.999 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
