6UKZ
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.440, 109.130, 59.030 |
| Unit cell angles | 90.00, 95.41, 90.00 |
Refinement procedure
| Resolution | 22.250 - 1.520 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6UKM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data scaling software | Aimless (0.6.2) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.278 | 1.548 |
| High resolution limit [Å] | 1.520 | 1.522 |
| Rmerge | 0.027 | 0.493 |
| Rmeas | 0.031 | 0.481 |
| Rpim | 0.017 | 0.254 |
| Total number of observations | 35570 | |
| Number of reflections | 67964 | 10157 |
| <I/σ(I)> | 20.2 | 2.5 |
| Completeness [%] | 96.9 | 99.6 |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 1.000 | 0.853 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
