6UKA

Crystal structure of RHOG and ELMO complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-09
Detector	Bruker PHOTON II
Wavelength(s)	1.34165
Spacegroup name	C 1 2 1
Unit cell lengths	100.279, 40.060, 73.930
Unit cell angles	90.00, 119.71, 90.00

Refinement procedure

Resolution	27.010 - 2.400
R-factor	0.1909
Rwork	0.186
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a2b
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.010	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.113	0.259
Number of reflections	38996	466
<I/σ(I)>	13.42	5.4
Completeness [%]	93.9	89.2
Redundancy	5.7	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M CHES, 0.95M sodium citrate