6UK0

HIV-1 M184V reverse transcriptase-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97741
Spacegroup name	C 2 2 21
Unit cell lengths	167.982, 169.682, 97.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.684 - 2.757
R-factor	0.208076918245
Rwork	0.205
R-free	0.26007
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kjv
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.083	0.491
Number of reflections	35981	3384
<I/σ(I)>	16.1
Completeness [%]	99.9
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2% PEG 4000, 100mM MES pH 6.0, 10mM magnesium sulfate