6UJY
HIV-1 wild-type reverse transcriptase-DNA complex with (-)-3TC-TP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97395 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 166.702, 169.479, 102.766 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.457 - 2.595 |
| R-factor | 0.17995419765 |
| Rwork | 0.177 |
| R-free | 0.23354 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kk1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.186 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.595 | 2.600 |
| Rmerge | 0.068 | 0.497 |
| Number of reflections | 45025 | 2238 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% PEG 4000, 100mM MES pH 6.0, 10mM magnesium sulfate |
