6UJY
HIV-1 wild-type reverse transcriptase-DNA complex with (-)-3TC-TP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97395 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 166.702, 169.479, 102.766 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.457 - 2.595 |
R-factor | 0.17995419765 |
Rwork | 0.177 |
R-free | 0.23354 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kk1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.186 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.595 | 2.600 |
Rmerge | 0.068 | 0.497 |
Number of reflections | 45025 | 2238 |
<I/σ(I)> | 16.5 | |
Completeness [%] | 99.7 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2% PEG 4000, 100mM MES pH 6.0, 10mM magnesium sulfate |