6UIQ
Crystal structure of wild-type human phosphoglucomutase 1 in complex with Glucose-6-Phosphate
Replaces: 6BJ0Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2017-09-23 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 171.874, 171.874, 99.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.810 - 2.300 |
R-factor | 0.170935744974 |
Rwork | 0.168 |
R-free | 0.22252 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5epc |
RMSD bond length | 0.007 |
RMSD bond angle | 0.893 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.820 | 60.820 | 2.360 |
High resolution limit [Å] | 2.300 | 10.540 | 2.300 |
Rmerge | 0.160 | 0.035 | 1.774 |
Rmeas | 0.167 | 0.036 | 1.868 |
Rpim | 0.045 | 0.010 | 0.573 |
Total number of observations | 878402 | 10241 | 37536 |
Number of reflections | 64976 | 809 | 3790 |
<I/σ(I)> | 14.8 | 51.5 | 1.3 |
Completeness [%] | 97.8 | 99.4 | 82.6 |
Redundancy | 13.5 | 12.7 | 9.9 |
CC(1/2) | 0.998 | 0.999 | 0.656 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350 |