6UHY

WDR5 in complex with Myc site fragment inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-06
Detector	MAR CCD 165 mm
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	134.464, 46.955, 112.770
Unit cell angles	90.00, 117.63, 90.00

Refinement procedure

Resolution	31.297 - 1.260
R-factor	0.1554
Rwork	0.154
R-free	0.18140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.280
High resolution limit [Å]	1.260	1.260
Rpim	0.035
Number of reflections	162176	7904
<I/σ(I)>	33.4
Completeness [%]	96.2
Redundancy	4.1
CC(1/2)		0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Bis-Tris pH 6.0, 0.2 M ammonium acetate, 28% to 32% PEG3350.