6UG8
Complex of ch28/11 Fab and SSEA-4 (monoclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.464, 69.167, 97.550 |
| Unit cell angles | 90.00, 94.46, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.890 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ug7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.908 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmeas | 0.070 | 0.570 |
| Number of reflections | 39580 | 6187 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, sodium cacodylate, magnesium acetate |
