6UEE
Pseudomonas aeruginosa LpxA Complex Structure with Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-10-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.310, 82.560, 221.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.320 - 2.100 |
| R-factor | 0.1851 |
| Rwork | 0.182 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.493 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.330 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.094 | 0.498 |
| Number of reflections | 71637 | 10722 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 82.6 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20% PEG 1000 0.2M calcium acetate 0.1M imidazole pH 7.0 |
