6UE0
Crystal structure of dihydrodipicolinate synthase from Klebsiella pneumoniae bound to pyruvate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.634, 114.230, 55.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.990 - 1.892 |
| Rwork | 0.169 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yxc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.039 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 54112 | 3259 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.7 | 95.3 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris, ph 7.5, 2 M Ammonium Sulfate, 5 mM Pyruvate |
