6UCQ

Crystal structure of the Thermus thermophilus 70S ribosome recycling complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	209.655, 448.741, 623.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.890 - 3.500
R-factor	0.2464
Rwork	0.244
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.890	49.890	3.710
High resolution limit [Å]	3.500	10.480	3.500
Rmerge	0.330	0.043	2.779
Rmeas	0.372	0.049	3.130
Total number of observations	3383227
Number of reflections	725815	27987	114996
<I/σ(I)>	4.48	27.29	0.64
Completeness [%]	98.7	98.2	97.3
Redundancy	4.661	4.497	4.681
CC(1/2)	0.996	0.996	0.152

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.3	292	0.15 M L-arginine-HCl, 0.1M Tris-HCl pH 7.3, 2.4-2.7% PEG-20K, 7-9% MPD, 0.5 mM BME