6UCA

Crystal structure of human ZCCHC4 in complex with SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.283
Spacegroup name	P 21 21 2
Unit cell lengths	136.567, 290.380, 83.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.683 - 3.103
R-factor	0.25
Rwork	0.249
R-free	0.28130
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	CRANK2
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.210
High resolution limit [Å]	3.100	6.670	3.100
Rmerge	0.126	0.066	0.752
Rmeas	0.139	0.073	0.831
Rpim	0.057	0.030	0.349
Number of reflections	60780	6431	5987
<I/σ(I)>	6
Completeness [%]	99.8	99.7	99.9
Redundancy	5.7	5.4	5.5
CC(1/2)		0.996	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	285	0.03 M citric acid, 0.07 M Bis-Tris propane, pH 7.6, 11-12% PEG3350, 10% glycerol, 5 mM TCEP, pH 7.0