6UBV

The structure of the Streptococcus gordonii surface protein SspB in complex with TEV peptide provides clues to the adherence of oral streptococcal adherence to salivary agglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-07-05
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	65.810, 132.697, 243.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 2.700
R-factor	0.2086
Rwork	0.206
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tzl
Data reduction software	DIALS (1.10.0)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.725	2.780
High resolution limit [Å]	2.700	2.700
Rmerge	0.108	0.419
Rmeas	0.123	0.530
Rpim	0.058	0.321
Number of reflections	56119	3400
<I/σ(I)>	11.6	1.7
Completeness [%]	94.0	71.3
Redundancy	4.2	2
CC(1/2)	0.992	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG 4000, 650 mM lithium sulfate, 50 mM Tris pH 7.5