6U8K

Crystal structure of hepatitis C virus IRES junction IIIabc in complex with Fab HCV3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-02-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 43
Unit cell lengths	173.289, 173.289, 140.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	173.289 - 2.750
R-factor	0.1871
Rwork	0.186
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Fab HCV2 6u8d
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	173.289	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.147	1.072
Rmeas	0.165
Rpim	0.073	0.539
Number of reflections	106558	10622
<I/σ(I)>	9.31	1.11
Completeness [%]	98.5	99.03
Redundancy	4.7	4.8
CC(1/2)	0.965	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1 M HEPES pH 7.5, 1.4 M sodium citrate tribasic dihydrate