6U8K
Crystal structure of hepatitis C virus IRES junction IIIabc in complex with Fab HCV3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-02-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 43 |
Unit cell lengths | 173.289, 173.289, 140.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 173.289 - 2.750 |
R-factor | 0.1871 |
Rwork | 0.186 |
R-free | 0.22240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Fab HCV2 6u8d |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 173.289 | 2.848 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.147 | 1.072 |
Rmeas | 0.165 | |
Rpim | 0.073 | 0.539 |
Number of reflections | 106558 | 10622 |
<I/σ(I)> | 9.31 | 1.11 |
Completeness [%] | 98.5 | 99.03 |
Redundancy | 4.7 | 4.8 |
CC(1/2) | 0.965 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M HEPES pH 7.5, 1.4 M sodium citrate tribasic dihydrate |