6U82
Crystal Structure of the Double Homeodomain of DUX4 in Complex with a DNA aptamer containing bulge and loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.320, 75.670, 82.690 |
| Unit cell angles | 90.00, 121.60, 90.00 |
Refinement procedure
| Resolution | 60.870 - 3.210 |
| R-factor | 0.2531 |
| Rwork | 0.252 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e8c |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.478 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.870 | 3.400 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.073 | 1.270 |
| Number of reflections | 10373 | 1874 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.997 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Magnesium acetate, PEG8000, Sodium cacodylate, pH 6.5 |
