6U81
Crystal Structure of the Double Homeodomain of DUX4 in Complex with a DNA aptamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 71.640, 73.190, 108.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.270 - 2.340 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.24700 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 6e8c |
RMSD bond length | 0.005 |
RMSD bond angle | 0.693 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.010 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 1.700 | |
Number of reflections | 12667 | 1897 |
<I/σ(I)> | 0.083 | |
Completeness [%] | 96.3 | |
Redundancy | 3.6 | |
CC(1/2) | 0.999 | 0.390 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Ethylene glycols, Glycerol, PEG4000, Imidazole, MES, pH 6.5 |