6U81

Crystal Structure of the Double Homeodomain of DUX4 in Complex with a DNA aptamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	71.640, 73.190, 108.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.270 - 2.340
R-factor	0.2177
Rwork	0.216
R-free	0.24700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6e8c
RMSD bond length	0.005
RMSD bond angle	0.693
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.010	2.390
High resolution limit [Å]	2.310	2.310
Rmerge		1.700
Number of reflections	12667	1897
<I/σ(I)>	0.083
Completeness [%]	96.3
Redundancy	3.6
CC(1/2)	0.999	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Ethylene glycols, Glycerol, PEG4000, Imidazole, MES, pH 6.5