6U66
Structure of the trimeric globular domain of Adiponectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.923, 49.635, 50.306 |
| Unit cell angles | 116.83, 100.89, 104.92 |
Refinement procedure
| Resolution | 40.768 - 0.990 |
| R-factor | 0.1657 |
| Rwork | 0.165 |
| R-free | 0.18950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dou |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 40.768 | 1.050 |
| High resolution limit [Å] | 0.989 | 2.960 | 0.990 |
| Rmerge | 0.134 | 0.037 | 1.545 |
| Rmeas | 0.155 | 0.042 | |
| Number of reflections | 199981 | 7683 | 31395 |
| <I/σ(I)> | 5.01 | 30.63 | 0.32 |
| Completeness [%] | 95.6 | 98.1 | 92.9 |
| Redundancy | 3.95 | 3.978 | 3.909 |
| CC(1/2) | 0.997 | 0.998 | 0.248 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.2 mM Li2SO4, 0.1 M sodium acetate pH 4.5, 50% PEG 400 |
