6U58

Toho1 Beta Lactamase Glu166Gln Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	293
Detector technology	PIXEL
Collection date	2019-05-01
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	73.543, 73.543, 99.721
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.420 - 1.890
Rwork	0.240
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xqz
Data reduction software	CrysalisPro
Refinement software	PHENIX ((1.14_3260))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.340	1.940	1.960
High resolution limit [Å]	1.900	1.900	1.890
Number of reflections	133121	1660	2136
<I/σ(I)>	11.4
Completeness [%]	100.0
Redundancy	5.3
CC(1/2)	0.995	0.635	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293
1	BATCH MODE		293