6U3W
Crystal structure of yeast alpha/epsilon-COP of the COPI vesicular coat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9782 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.264, 84.594, 117.842 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.460 - 2.394 |
R-factor | 0.1824 |
Rwork | 0.178 |
R-free | 0.23780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mv2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.884 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.440 |
High resolution limit [Å] | 2.390 | 6.500 | 2.390 |
Rmerge | 0.104 | 0.054 | 0.673 |
Rmeas | 0.114 | 0.060 | 0.767 |
Rpim | 0.047 | 0.025 | 0.360 |
Number of reflections | 29047 | 1591 | 1404 |
<I/σ(I)> | 5.1 | ||
Completeness [%] | 99.7 | 99.7 | 98.1 |
Redundancy | 5.6 | 5.2 | 4.4 |
CC(1/2) | 0.996 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris, pH 8.5, 0.2 M Li2SO4, 15% (w/v) PEG 3350, 25% (v/v) glycerol Cryoprotected with 23% (w/v) PEG 3350, 25% (v/v) glycerol |