6U3A
1.65 Angstrom crystal structure of the N97S Ca-CaM:CaV1.2 IQ domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.419, 68.077, 86.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.661 - 1.650 |
R-factor | 0.1795 |
Rwork | 0.178 |
R-free | 0.20880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2be6 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 35.661 | 1.750 |
High resolution limit [Å] | 1.650 | 4.930 | 1.650 |
Number of reflections | 85584 | 3173 | 13835 |
<I/σ(I)> | 13.5 | ||
Completeness [%] | 98.6 | ||
Redundancy | 2.93 | ||
CC(1/2) | 0.999 | 0.999 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | DL-malic acid |