6U2U
Helix-Loop-helix motif of mouse DNA-binding protein inhibitor ID-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.95000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.893, 57.712, 32.097 |
Unit cell angles | 90.00, 114.29, 90.00 |
Refinement procedure
Resolution | 29.256 - 1.500 |
R-factor | 0.2059 |
Rwork | 0.202 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mgm |
RMSD bond length | 0.006 |
RMSD bond angle | 0.803 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmeas | 0.234 | |
Rpim | 0.028 | |
Number of reflections | 13599 | 1182 |
<I/σ(I)> | 35.9 | |
Completeness [%] | 98.1 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 299 | 0.1 M MES (pH 6.5), 0.2 M sodium acetate |