6U08

Double-stranded DNA-specific cytidine deaminase type VI secretion system effector and cognate immunity complex from Burkholderia cenocepacia

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-19
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	126.760, 144.953, 64.186
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.603 - 2.491
R-factor	0.1745
Rwork	0.172
R-free	0.22750
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.603	2.530
High resolution limit [Å]	2.491	2.491
Rmerge	0.120
Number of reflections	78988	1339
<I/σ(I)>	32.7
Completeness [%]	98.9	100
Redundancy	13	12.6
CC(1/2)		0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.1 M Bis-Tris:Hcl pH 6.5, 200 mM MgCl2